Coherent Anti-Stokes Raman Scattering Microscopy: Chemical Imaging for Biology and Medicine: Supplemental Video 1

A supplemental video from the 2008 review by Conor L. Evans and X. Sunney Xie, "Coherent Anti-Stokes Raman Scattering Microscopy: Chemical Imaging for Biology and Medicine," from the Annual Review of Analytical Chemistry.

Video demonstrating the ability of coherent anti-Stokes Raman scattering (CARS) microscopy to image the human retina. The movie shows a 100x magnification, 180 μm × 180 μm, 55-μm forward propagating CARS (F-CARS) depth stack through an ex vivo human retina at the lipid band. The animation begins at the retina surface, where a capillary can be seen on top of the nerve fiber layer. As the depth increases, each layer of the retina can be seen, with the photoreceptor layer imaged at a final depth of 55 μm.