The Formation of TGN-to-Plasma-Membrane Transport Carriers

In the trans-Golgi network (TGN), proteins are sorted for transport to the endosomes, plasma membrane, preceding Golgi cisternae, and endoplasmic reticulum. The formation of clathrin-coated vesicles for transport to the endosomes and of COP-I-coated vesicles for retrograde trafficking is fairly well characterized at the molecular level. We describe our current understanding of the TGN-to-cell-surface carriers, with a specific focus on the components involved in membrane fission. Inhibiting the fission machinery promotes growth of transport carriers into large tubules that remain attached to the TGN. Overactivating this machinery, on the other hand, vesiculates the TGN. To understand how membrane fission is regulated by cargo to form transport carriers yet prevents complete vesiculation of the TGN remains a daunting challenge. We discuss these issues with regard to TGN-to-cell-surface transport carriers.