Spatiotemporal Basis of Innate and Adaptive Immunity in Secondary Lymphoid Tissue: Video 2

A video from the 2014 review by Hai Qi, Wolfgang Kastenmüller, and Ronald N. Germain, "Spatiotemporal Basis of Innate and Adaptive Immunity in Secondary Lymphoid Tissue," from the Annual Review of Cell and Developmental Biology.

Shown: Congregating neutrophils displace fibrous collagen bundles from the wound center

This video shows the collagenous fiber network (visualized by second harmonic generation) as neutrophils accumulate at the focal damage site in the dermis of a LysM^gfp/+Tyr^c-2J/c-2J mouse. Fiber bundles are displaced over time in the x-y axis. Some disappearing fibers are also displaced in the z-axis out of the imaging volume (not shown).

Original publication: Lämmermann T, Afonso PV, Angermann BR, Wang JM, Kastenmuller W, et al. 2013. Neutrophil swarms require LTB4 and integrins at sites of cell death in vivo. Nature 498:371–75