Spatiotemporal Basis of Innate and Adaptive Immunity in Secondary Lymphoid Tissue: Video 6

A video from the 2014 review by Hai Qi, Wolfgang Kastenmüller, and Ronald N. Germain, "Spatiotemporal Basis of Innate and Adaptive Immunity in Secondary Lymphoid Tissue," from the Annual Review of Cell and Developmental Biology.

Shown: LTB4 requirement for swarming in infected lymph nodes

Mice were infected with P. aeruginosa-GFP in the footpad before 2P-IVM was performed on the draining popliteal lymph nodes when comparable neutrophil numbers were present in the subcapsular sinus at indicated times after infection (WT: 3 h, Ltb4r1^−/−: 4.5 h). Neutrophil-GFP signal is pseudo-colored (heat map) to indicate neutrophil clusters (white) in WT-LysM^gfp/+ mice (left) and Ltb4r1^−/−LysM^gfp/+ mice (right).

Original publication: Lämmermann T, Afonso PV, Angermann BR, Wang JM, Kastenmuller W, et al. 2013. Neutrophil swarms require LTB4 and integrins at sites of cell death in vivo. Nature 498:371–75