Spatiotemporal Basis of Innate and Adaptive Immunity in Secondary Lymphoid Tissue: Video 10

A video from the 2014 review by Hai Qi, Wolfgang Kastenmüller, and Ronald N. Germain, "Spatiotemporal Basis of Innate and Adaptive Immunity in Secondary Lymphoid Tissue," from the Annual Review of Cell and Developmental Biology.

Shown: (a) Kinetics of naive CD8⁺ T cell priming

Popliteal LNs from MVA OVA-infected (footpad) animals were surgically exposed and analyzed by two-photon microscopy. Prior (24 h) to infection mice received labeled (CMFDA) naive (CD44^lo) OT-I T cells. Video shows the dynamic behavior and cluster formation of OT-I T cells in the course of infection as well as the positioning of the clusters in relation to HEV.

(b) Migrational arrest of antigen-specific CD8⁺ T cells after infection

Popliteal LNs from MVA OVA-infected (footpad) animals were surgically exposed and analyzed by two-photon microscopy. Prior (24 h) to infection mice received labeled (CMFDA) naive (CD44^lo) OT-I T cells and labeled (CTR) naive (CD44^lo) polyclonal control T cells. Video shows migrational arrest of OT-I and active migration of nonspecific polyclonal CD8⁺ T cells.

Original publication: Kastenmuller W, Brandes M, Wang Z, Herz J, Egen JG, Germain RN. 2013. Peripheral prepositioning and local CXCL9 chemokine-mediated guidance orchestrate rapid memory CD8⁺ T cell responses in the lymph node. Immunity 38:502–13