Spatiotemporal Basis of Innate and Adaptive Immunity in Secondary Lymphoid Tissue: Video 11

A video from the 2014 review by Hai Qi, Wolfgang Kastenmüller, and Ronald N. Germain, "Spatiotemporal Basis of Innate and Adaptive Immunity in Secondary Lymphoid Tissue," from the Annual Review of Cell and Developmental Biology.

Shown: CCR5 guides naive CD8⁺ T cells to helped DC

Video illustrates differences in the contact frequency between CD4⁺ T cell helped (blue) DCs and each of the two polyclonal CD8⁺ T cell populations. 57 contacts are made between CD4⁺ T cell helped DCs and WT CD8⁺ T cells (red dots and circles, left panel) as compared to 6 contacts between the same DCs and CCR5 ^−/− CD8⁺ T cells (green dots and circles, right panel), resulting in a calculated hit rate ratio of 3.26 for WT versus CCR5 ^−/− CD8⁺ T cells interacting with DCs.

Original publication: Castellino F, Huang AY, Altan-Bonnet G, Stoll S, Scheinecker C, Germain RN. 2006. Chemokines enhance immunity by guiding naive CD8⁺ T cells to sites of CD4⁺ T cell-dendritic cell interaction. Nature 440:890–95