Spatiotemporal Basis of Innate and Adaptive Immunity in Secondary Lymphoid Tissue: Video 17

A video from the 2014 review by Hai Qi, Wolfgang Kastenmüller, and Ronald N. Germain, "Spatiotemporal Basis of Innate and Adaptive Immunity in Secondary Lymphoid Tissue," from the Annual Review of Cell and Developmental Biology.

Shown: Sap ^−/− T cells fail to be recruited and retained in nascent GC

CFP-expressing Sap ^+/+ OT-2 T cells (red), GFP-expressing Sap ^−/− OT-2 T cells (green), and non-fluorescent MD4 B cells were cotransferred into mice that were subsequently immunized with HEL-OVA. Intravital imaging of draining LNs was performed six to eight days post immunization. CMTPX-labeled polyclonal naïve B cells (blue) were transferred one or two days prior to imaging to help demarcate follicular mantle and GC. The dotted line at the beginning of the video approximates the GC border in this maximum intensity projection.

Original publication: Qi H, Cannons JL, Klauschen F, Schwartzberg PL, Germain RN. 2008. SAP-controlled T-B cell interactions underlie germinal centre formation. Nature 455:764–69